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Mouse Anti-Vimentin/AP Conjugated antibody (bsm-33170M-AP)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bsm-33170M-AP
英文名稱1 Mouse Anti-Vimentin/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標(biāo)記的波形蛋白單克隆抗體
別    名 FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領(lǐng)域 腫瘤  細(xì)胞生物  發(fā)育生物學(xué)  信號轉(zhuǎn)導(dǎo)  干細(xì)胞  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 3B7
交叉反應(yīng) Human,  (predicted: Mouse, Rat, )
產(chǎn)品應(yīng)用 WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 53kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human Vimentin Protein
亞    型 IgG
純化方法 affinity purified by Protein G
儲 存 液 Constituents: 0.05M TBS, pH 8.0 with 10mg/ml BSA and 0.05% NaN3, 50% glycerol. Or Lyophilized. Buffer = 0.05M TBS, pH 8.0 with 10mg/ml BSA and 0.05% NaN3. Reconstitute with sterile distilled water.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Function:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Subunit:
Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B (By similarity).

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Post-translational modifications:
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.

Similarity:
Belongs to the intermediate filament family.

Database links:

Entrez Gene: 7431 Human

Entrez Gene: 22352 Mouse

Entrez Gene: 81818 Rat

Omim: 193060 Human

SwissProt: P08670 Human

SwissProt: P20152 Mouse

SwissProt: P31000 Rat

Unigene: 455493 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Vimentin—波形蛋白。是五種主要的中間絲之一,存在于各種正常和病理性間質(zhì)來源的組織,如纖維母細(xì)胞、內(nèi)皮細(xì)胞、淋巴細(xì)胞等正常細(xì)胞和肉瘤、淋巴瘤、黑色素瘤等腫瘤。波形蛋白是負(fù)責(zé)維持細(xì)胞骨架完整性的蛋白之一。
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