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Rabbit
Anti-Rb/PE-Cy7
Cat. Number:
bs-2777R-PE-Cy7
Quantity size:
100ul
Concentration:
1mg/ml Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Background:
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
Also known as:
Including osteosarcoma;
OSRC;
Osteosarcoma;
P105 RB;
p105-Rb;
P105RB;
PP105;
PP110;
PPP1R130;
pRb;
pRb;
Prepro retinoblastoma associated protein;
Protein phosphatase 1 regulatory subunit 130;
RB 1;
Rb;
RB_HUMAN;
RB1;
RB1 gene;
RB1 protein;
Retinoblastoma 1 (including osteosarcoma);
Retinoblastoma 1;
Retinoblastoma 1;
Retinoblastoma associated protein;
Retinoblastoma related osteosarcoma;
Retinoblastoma susceptibility gene;
Retinoblastoma suspectibility protein;
Retinoblastoma-associated protein.
Specificity:
●
Rabbit Polyclonal IgG, affinity purified by Protein A.
●
Reacts with:
(predicted: )
●
Immunogen: KLH conjugated synthetic peptide derived from human P105 RB.
●
Predicted Molecular Weight: 102kDa.
Storage:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Application:
Excitation spectrum: 488nm,561nm,743nm
Emission spectrum: 785nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.