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Rabbit Anti-TIMP-1/AP Conjugated antibody (bs-0415R-AP)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-0415R-AP
英文名稱1 Rabbit Anti-TIMP-1/AP Conjugated antibody
中文名稱 堿性磷酸酶(AP)標(biāo)記的金屬蛋白酶組織抑制因子-1抗體
別    名 Clgi; Collagenase inhibitor; EPA; EPO; Erythroid Potentiating Activity; Fibroblast collagenase inhibitor; FLJ90373; HC; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP1 protein; Tissue Inhibitor of Metalloproteinase 1; Tissue inhibitor of metalloproteinases; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 腫瘤  細(xì)胞生物  神經(jīng)生物學(xué)  細(xì)胞凋亡  生長(zhǎng)因子和激素  激酶和磷酸酶  合成與降解  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, )
產(chǎn)品應(yīng)用 WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 21kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TIMP-1
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq].

Function:
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

Subcellular Location:
Secreted.

Post-translational modifications:
The activity of TIMP1 is dependent on the presence of disulfide bonds.

Similarity:
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.

Database links:

Entrez Gene: 7076 Human

Entrez Gene: 21857 Mouse

Omim: 305370 Human

SwissProt: P01033 Human

SwissProt: P12032 Mouse

Unigene: 522632 Human

Unigene: 8245 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

合成與降解(Synthesis and Degradation)
TIMP1是基質(zhì)金屬蛋白酶的抑制酶,其功能與MMP相反,能抑制腫瘤細(xì)胞的浸潤(rùn)和轉(zhuǎn)移,主要用于各種惡性腫瘤如乳腺癌等的研究。TIMP-1是一種28kDa的糖蛋白,主要分泌細(xì)胞為多種結(jié)締組織細(xì)胞,被認(rèn)為是組織中MMPs活性的主要調(diào)節(jié)者,它可以和MMPs家族成員以共價(jià)鍵方式形成1:1的復(fù)合物,從而發(fā)揮其抑制作用。在正常生理?xiàng)l件下,MMPs和TIMPs之間的平衡狀態(tài)是維持細(xì)胞外基質(zhì)正常狀態(tài)所必需的,一旦它們之間的比例失調(diào),就會(huì)造成一系列的病理狀態(tài)。
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