| 產(chǎn)品編號 | bs-0701R-RBITC |
| 英文名稱 | Rabbit Anti-Ceramide glucosyltransferase /RBITC Conjugated antibody |
| 中文名稱 | 羅丹明（RBITC）標記的谷氨酸半胱氨酸γ合成酶抗體 |
| 別 名 | CEGT_HUMAN; UGCG; GCS; GLCT 1; GLCT-1; GLCT1; Glucosylceramide synthase; UDP glucose ceramide glucosyltransferase; UDP glucose N acylsphingosine D glucosyltransferase; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase; Ugcg. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Cow, ) |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 44kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: GCS (glucosylceramide synthase ) may serve as a 'flippase' as well as a glucosyltransferase that transfers glucose to ceramide. Able to use UDP-galactose to synthesize galactosylceramide with 10% of efficiency with which it utilizes UDP-glucose. [Catalytic activity] UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Lipid metabolism; sphingolipid metabolism. [Subcellular location] Endoplasmic reticulum membrane; Multi-pass membrane protein. Belongs to the glycosyltransferase 2 family. Function: Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Found in all tissues examined. Similarity: Belongs to the glycosyltransferase 2 family. Database links: Entrez Gene: 7357 Human Entrez Gene: 22234 Mouse Omim: 602874 Human SwissProt: Q16739 Human SwissProt: O88693 Mouse Unigene: 304249 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 谷氨酸半胱氨酸γ合成酶又稱葡萄糖神經(jīng)酰胺合成酶(glucosylceramide synthase, GCS)是調(diào)控神經(jīng)酰胺代謝的關(guān)鍵酶之一, 其活性水平與腫瘤細胞多藥耐藥有密切關(guān)系. 有研究報道GCS在人卵巢癌細胞、口腔表皮癌細胞 、黑色素瘤細胞、乳腺癌細胞及膽囊癌、胃癌中均有表達. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
