| 產品編號 | bs-3996R-BF647 |
| 英文名稱1 | Rabbit Anti-MDH1/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的可溶性蘋果酸脫氫酶抗體 |
| 別 名 | Cytosolic malate dehydrogenase; Malate dehydrogenase 1, NAD (soluble); MDHA; MDHs; Soluble malate dehydrogenase; Malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1; Malate dehydrogenase 1 NAD (soluble); Malate dehydrogenase 2; Malate dehydrogenase cytoplasmic; Malate dehydrogenase 2 NAD (mitochondrial); MDH 1; MDH 2; MDH; MDH1; MDH2; MDHA; MDHm; MDHs; MMDH; Mor 1; Mor 2; Mor1; MOR2; cytoplasmic; MDH s; mdh1; MDHC_HUMAN; MGC:1375; SMDH; Soluble malate dehydrogenase. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產品應用 | Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MDH1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. Malate dehydrogenase 1 (MDH1) is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Subunit: Homodimer. Subcellular Location: Cytoplasm. Post-translational modifications: ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation. Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family. Database links: Entrez Gene: 4190 Human Entrez Gene: 17449 Mouse Omim: 154200 Human SwissProt: P40925 Human SwissProt: P14152 Mouse Unigene: 526521 Human Unigene: 212703 Mouse Unigene: 13492 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control: U937.
Primary Antibody (green line): Rabbit Anti-BRN3A antibody (bs-3996R-BF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
