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Rabbit Anti-IDH2/PE-Cy3 Conjugated antibody (bs-3947R-PE-Cy3)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-3947R-PE-Cy3
英文名稱1 Rabbit Anti-IDH2/PE-Cy3 Conjugated antibody
中文名稱 PE-Cy3標(biāo)記的草酰琥珀酸脫羧酶抗體
別    名 mitochondrial; ICD-M; IDH; IDH2; IDHP_HUMAN; IDP; Isocitrate dehydrogenase [NADP]; Isocitrate dehydrogenase [NADP], mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 腫瘤  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號(hào)  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Rat,  (predicted: Mouse, Dog, Horse, )
產(chǎn)品應(yīng)用 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 51kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IDH2
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit.

Function:
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

Subunit:
Homodimer.

Subcellular Location:
Cytoplasm. Peroxisome.

DISEASE:
Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Similarity:
Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Database links:

Entrez Gene: 3418 Human

Entrez Gene: 269951 Mouse

Entrez Gene: 361596 Rat

Omim: 147650 Human

SwissProt: P48735 Human

SwissProt: P54071 Mouse

SwissProt: P56574 Rat

Unigene: 596461 Human

Unigene: 246432 Mouse

Unigene: 290925 Mouse

Unigene: 3490 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

IDH2又稱異檸檬酸脫氫酶,他有有兩種類型的酶:以NAD為輔酶的酶(EC1.1.1.41)和NADP的酶(EC1.1.1.42),兩者催化同一反應(yīng)。在胞漿中以NADP為輔酶,在線粒體內(nèi)膜以NAD為輔酶。    IDH2在三羧酸循環(huán)中正常起作用的是NAD為輔酶的酶,他是提供為ADP激活的變構(gòu)酶。
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