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Rabbit Anti-Cryptochrome 1/PE-Cy5 Conjugated antibody (bs-11441R-PE-Cy5)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-11441R-PE-Cy5
英文名稱 Rabbit Anti-Cryptochrome 1/PE-Cy5 Conjugated antibody
中文名稱 PE-Cy5標記的隱花色素蛋白1抗體
別    名 Cryptochrome I; CRY1; CRY1_HUMAN; Cryptochrome 1 (photolyase like); Cryptochrome 1; Cryptochrome-1; Cryptochrome1; PHLL1; Photolyase-like.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  神經生物學  信號轉導  轉錄調節(jié)因子  通道蛋白  細胞膜受體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,  (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, )
產品應用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 64kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Cryptochrome 1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
Circadian clocks are biological timepieces that regulate hormonal rhythms, sleep cycles and feeding behaviors. These rhythms are generated in the superchiasmatic nucleus (SCN), a cell-autonomous circadian oscillator located within the brain that is synchronized with the environment by light. A number of transcription factors, including Clock and BMAL1, are molecular components of the SCN that induce the expression of proteins involved in light/dark cycle entrainment, which include Per1 and Per2. Tim, for timeless, generates a negative feedback loop that regulates the activity of Clock by suppressing the expression of Clock target genes. Tim forms heterodimers with Per1 and Per2 that bind Clock and block the activation of Clock-BMAL1 dimers to repress Per gene expression. Additionally, the CRY proteins, which are cryptochrome photoreceptors for the circadian clock, function as light-independent inhibitors of the circadian clock. CRY1 and CRY2 negatively regulate SCN components by associating with the activators Clock-BMAL1, and also with the various feedback inhibitors Per1, Per2 and Tim.

Function:
Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK.

Subunit:
Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Binds MAPK. Interacts with FBXL21 (By similarity). Interacts with FBXL3.

Subcellular Location:
Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.

Post-translational modifications:
Phosphorylation on Ser-247 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSNK1E requires interaction with PER1 or PER2. Phosphorylation at Ser-71 and Ser-280 by AMPK destabilizes it.
Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex leading to degradation.

Similarity:
Belongs to the DNA photolyase class-1 family.
Contains 1 DNA photolyase domain.

Database links:

Entrez Gene: 1407 Human

Omim: 601933 Human

SwissProt: Q16526 Human

Unigene: 151573 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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