| 產(chǎn)品編號 | bs-1982R-PE |
| 英文名稱 | Rabbit Anti-MCM3/PE Conjugated antibody |
| 中文名稱 | PE標記的微小染色體維持缺陷蛋白3抗體 |
| 別 名 | minichromosome maintenance deficient 3; Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC 5; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MCM 3; MCM3 minichromosome maintenance deficient 3; MGC1157; Minichromosome maintenance deficient 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P1.h; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta; RLFB. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 染色質(zhì)和核信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) |
| 產(chǎn)品應用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 89kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MCM3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] Subunit: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP. Subcellular Location: Nucleus. Similarity: Belongs to the MCM family. Contains 1 MCM domain. Database links: Entrez Gene: 4172 Human Entrez Gene: 17215 Mouse Omim: 602693 Human SwissProt: P25205 Human SwissProt: P25206 Mouse Unigene: 179565 Human Unigene: 4502 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. MCM3也是MCMs1-7個家族中重要的一員,與生物體DNA復制過程密切相關。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
