| 產(chǎn)品編號 | bs-2940R-AP |
| 英文名稱1 | Rabbit Anti-FUCA1/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標記的α-L巖藻糖苷酶抗體 |
| 別 名 | Alpha L fucosidase I; Alpha L fucoside fucohydrolase; Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; FUCA; FUCA1; FUCO_HUMAN; Tissue alpha L fucosidase; Tissue alpha-L-fucosidase. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FUCA1/Alpha L fucosidase I |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Alpha-L-fucosidase,AFU is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Function: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Subunit: Homotetramer. Subcellular Location: Lysosome. DISEASE: Fucosidosis (FUCA1D) [MIM:230000]: An autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the glycosyl hydrolase 29 family. Database links: Entrez Gene: 2517 Human Entrez Gene: 71665 Mouse Omim: 612280 Human SwissProt: P04066 Human SwissProt: Q99LJ1 Mouse Unigene: 370858 Human Unigene: 439940 Mouse Unigene: 3469 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. α-L-巖藻糖苷酶是一種催化含巖藻糖基的糖蛋白、糖脂等生物活性大分子水解酶的溶酶體酸性水解酶。其廣泛分布于人體組織細胞、血液和體液中。參與體內(nèi)糖蛋白、糖脂和寡糖的代謝。由于肝癌患者α-L-巖藻糖苷酶明顯升高，目前它被認為是原發(fā)性肝癌的一種新的腫瘤標記物。α-L巖藻糖苷酶升高：見于原發(fā)性肝癌、轉移性肝癌、肝硬化、急性肝炎等。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
