| 產(chǎn)品編號(hào) | bsm-55523R |
| 英文名稱 | MUC1/CD227 Recombinant Rabbit mAb |
| 中文名稱 | 粘蛋白-1重組兔單抗 |
| 別 名 | ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM, PEMT, Episialin, EAM, CA 15-3 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | SB48 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1: 500-2000,IHC-P=1: 200-1000,IHC-F=1: 200-1000,IF=1: 200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 性 狀 | Liquid |
| 免 疫 原 | Synthesized peptide derived from Human MUC1 |
| 亞 型 | IgG |
| 純化方法 | Affinity Purification |
| 緩 沖 液 | PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol. |
| 保存條件 | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation . MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail. MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation. MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail. Overexpression of MUC1 potentiates transformation and attenuates stress-induced apoptosis through the Akt or p53 pathways SWISS: P15941 Gene ID: 4582 Mucin（CD227）是糖蛋白家族的一員，以重復(fù)結(jié)構(gòu)域和密集的O-糖基化為特征。MUC1在呼吸道、胃腸道等所有上皮組織、皮脂腺和唾液腺以及造血細(xì)胞中都有表達(dá)，MUC1的非上皮性表達(dá)已被描述發(fā)生在白細(xì)胞上，包括T細(xì)胞、B細(xì)胞、骨髓單核細(xì)胞、單核細(xì)胞和樹突狀細(xì)胞（DCs）等。MUC1可進(jìn)行蛋白質(zhì)水解裂解，并且較大的胞外域與較小的羧基末端結(jié)構(gòu)域有關(guān)，后者包含一個(gè)跨膜片段和一個(gè)含72個(gè)殘基的胞質(zhì)尾區(qū)。MUC1對(duì)正常的上皮起潤(rùn)滑和保護(hù)作用，同時(shí)還介導(dǎo)信號(hào)轉(zhuǎn)導(dǎo)和細(xì)胞黏附功能，免疫活化和抑制作用等。在腫瘤組織中，MU |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MUC1 monoclonal antibody, unconjugated (bsm-55523R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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