| 產(chǎn)品編號(hào) | bsm-61087R |
| 英文名稱 | Galectin 3 Recombinant Rabbit mAb |
| 中文名稱 | 半乳糖凝集素3重組兔單抗 |
| 別 名 | Galectin-3; 35 kDa lectin; Carbohydrate binding protein 35; CBP 35; CBP35; GAL 3; GAL3; GAL-3; Galactose specific lectin 3; Galactose-specific lectin 3; Galactoside binding protein; Galactoside-binding protein; GALBP; Galectin3 internal gene, included; GA |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 5C5 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Galectin 3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
bs-0721P is one synthetic peptide derived from human Galectin 3. Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Subunit: Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. Subcellular Location: Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface. Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Similarity: Contains 1 galectin domain. SWISS: P17931 Gene ID: 3958 Database links: Entrez Gene: 3958 Human Entrez Gene: 16854 Mouse Omim: 153619 Human SwissProt: P17931 Human SwissProt: P16110 Mouse Unigene: 531081 Human Unigene: 248615 Mouse Unigene: 764 Rat Galectin-3是Galectin（半乳凝素）家族的一員，能識(shí)別糖蛋白和糖脂的特異性低聚糖結(jié)構(gòu)，參與多種生理和病理過(guò)程，包括細(xì)胞生長(zhǎng)和凋亡、細(xì)胞黏附、新生血管形成和腫瘤浸潤(rùn)與轉(zhuǎn)移等。Galectin-3在腫瘤的發(fā)生、發(fā)展及轉(zhuǎn)移中起重要作用。 Galectin-3在某些腫瘤組織中高表達(dá)，其在不同類型腫瘤，如結(jié)直腸癌、胃癌、胰腺癌、甲狀腺癌及肺癌等患者中的高表達(dá)水平，與低生存、腫瘤復(fù)發(fā)、預(yù)后差及對(duì)化療藥物敏感性降低相關(guān)對(duì)于某些腫瘤，Galectin-3有可能成為一個(gè)有價(jià)值的診斷標(biāo)志和治療靶點(diǎn)。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (bsm-61087R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Galectin 3 Monoclonal Antibody, Unconjugated(bsm-61087R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-BF594) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-BF594) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with Galectin 3 Monoclonal Antibody, Unconjugated (bsm-61087R) at 1:200 overnight at 4°C, followed by a BF594 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-BF594) for 90 minutes, and DAPI for nuclei staining.
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