| 產(chǎn)品編號 | bsm-61064R |
| 英文名稱 | APPL1 Recombinant Rabbit mAb |
| 中文名稱 | 銜接因子蛋白含pH域蛋白1重組兔單抗 |
| 別 名 | Adapter protein containing PH domain; Adaptor Protein Containing pH Domain; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1; Adaptor protein containing pH domain PTB domain and leucine zipper motif; AKT2 INTERACTOR; APPL 1; APPL |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 6F1 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APPL1 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008] Function: Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Subunit: Binds RAB5A/Rab5 through an N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Interacts with OCRL and INPP5B. Subcellular Location: Early endosome membrane; Peripheral membrane protein. Nucleus. Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. Tissue Specificity: High levels in heart, ovary, pancreas and skeletal muscle. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Phosophorylation at Ser-410 by PKA severely impairs binding to OCRL. Similarity: Contains 1 PH domain. Contains 1 PID domain. Database links: Entrez Gene: 26060 Human Entrez Gene: 72993 Mouse Omim: 604299 Human SwissProt: Q9UKG1 Human SwissProt: Q8K3H0 Mouse Unigene: 476415 Human Unigene: 202322 Mouse 銜接因子蛋白含pH域磷酸酪氨酸結(jié)合域和亮氨酸拉鏈基元1抗體. APPL1可調(diào)節(jié)脂聯(lián)素和胰島素信號，是細胞膜與細胞核之間重要的信息傳遞蛋白,同時也是細胞增殖所必需的蛋白，它有調(diào)節(jié)脂聯(lián)素在脂肪酸氧化和葡萄糖吸收中的作用，APPL1通過激酶通道來調(diào)節(jié)脂聯(lián)素的胰島素敏感效應(yīng)。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with APPL1 monoclonal antibody, unconjugated (bsm-61064R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated(bsm-61064R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated(bsm-61064R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated(bsm-61064R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated(bsm-61064R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with APPL1 Monoclonal Antibody, Unconjugated(bsm-61064R) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
