| 產(chǎn)品編號 | bsm-30251A |
| 英文名稱 | mouse CD45 Rat mAb |
| 中文名稱 | 小鼠CD45單克隆抗體 |
| 別 名 | LCA; T200 |
| 抗體來源 | Rat |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 8G5 |
| 交叉反應 | Mouse |
| 產(chǎn)品應用 | Flow-Cyt=1:50-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 143 kDa |
| 檢測分子量 | 220 |
| 性 狀 | Liquid |
| 亞 型 | Rat IgG2b, k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Enables several functions, including heparan sulfate proteoglycan binding activity; heparin binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including lymphocyte differentiation; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including lymphocyte differentiation; positive regulation of lymphocyte activation; and regulation of protein phosphorylation. Located in external side of plasma membrane; focal adhesion; and membrane raft. Is expressed in several structures, including 3rd branchial arch; alimentary system; cardiovascular system; hemolymphoid system; and placenta. Used to study systemic lupus erythematosus. Human ortholog(s) of this gene implicated in hepatitis C; multiple sclerosis; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive. Orthologous to human PTPRC (protein tyrosine phosphatase receptor type C). [provided by Alliance of Genome Resources, Apr 2022] |
| 產(chǎn)品圖片 |
The Mouse spleen cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rat Anti-CD45 antibody (bsm-30251A): 1 μg/10^6 cells; Secondary Antibody : Goat anti-Rat IgG-FITC: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
