| 產(chǎn)品編號(hào) | bsm-60471R |
| 英文名稱(chēng) | Rabbit Anti-HSPE1 antibody |
| 中文名稱(chēng) | 熱休克蛋白10/HSPE1重組兔單抗 |
| 別 名 | CH10_HUMAN; 10 kDa heat shock protein, mitochondrial; Hsp10; 10 kDa chaperonin; Chaperonin 10 (CPN10); Early-pregnancy factor (EPF); Early pregnancy factor; heat shock protein family E (Hsp10) member 1; Heat shock 10kD protein 1; mitochondrial; EPF; CPN10; GROES; HSP10; HSP 10; HSP-10; Cpn 10; HSPE 1; |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | F10C9 |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Cellular localization; Mitochondrion matrix. Function: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Subcellular Location: Mitochondrion matrix. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the GroES chaperonin family. SWISS: P61604 Gene ID: 3336 Database links: Entrez Gene: 3336 Human Entrez Gene: 15528 Mouse Omim: 600141 Human SwissProt: P61604 Human SwissProt: Q64433 Mouse Unigene: 1197 Human Unigene: 215667 Mouse Unigene: 106093 Rat |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate:1: MCF-7, 2: 293, 3: 4T1, 4: PC-12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60471R
Tissue: Rat colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60471R
Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60471R
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