| 產(chǎn)品編號(hào) | bsm-60444R |
| 英文名稱 | Rabbit Anti-ABCE1 antibody |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | A12F4 |
| 交叉反應(yīng) | Human (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 共翻譯質(zhì)量控制因子參與了不通過衰變（NGD）途徑。與PELO和HBS1L一起，是80S核糖體形成48S復(fù)合物和空位80S核糖體解離所必需的。與PELO和HBS1L一起識(shí)別停滯的核糖體并促進(jìn)組裝在不間斷mRNAs上的延伸復(fù)合物的解離，這觸發(fā)了mRNA的核內(nèi)裂解，這是一種釋放非功能性核糖體和降解受損mRNAs的機(jī)制，是No-Go衰變（NGD）途徑的一部分。在mRNA周轉(zhuǎn)的調(diào)控中起作用，在線粒體外膜定位mRNA翻譯的質(zhì)量控制中起作用。作為PINK1調(diào)控信號(hào)的一部分，在線粒體損傷時(shí)被CNOT4泛素化；這種修飾產(chǎn)生多泛素信號(hào)，將自噬受體招募到線粒體外膜并啟動(dòng)有絲分裂吞噬。RNASEL特異性蛋白抑制劑，拮抗2-5A（5'-磷酸化2'，5'-連接的寡腺苷酸）與RNASEL的結(jié)合。干擾素調(diào)節(jié)的2-5A/RNASEL途徑抗病毒作用的負(fù)調(diào)節(jié)因子。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1:HeLa, 2: Panc-1, 3: Mouse liver
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 67 kDa
Observed MW: 67 kDa
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60444R
Cell line: NIH-3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60444R
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