| 產(chǎn)品編號(hào) | bsm-60621R |
| 英文名稱(chēng) | Rabbit Anti-SMARCA2/BRM antibody |
| 中文名稱(chēng) | |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | E7F8 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000,IHC-P=1:20-100,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 通過(guò)染色質(zhì)重塑（DNA核小體拓?fù)浣Y(jié)構(gòu)的改變）參與選擇基因的轉(zhuǎn)錄激活和抑制。SWI/SNF染色質(zhì)重塑復(fù)合物的組成部分，執(zhí)行關(guān)鍵的酶活性，通過(guò)以ATP依賴(lài)的方式改變核小體內(nèi)DNA組蛋白的接觸來(lái)改變?nèi)旧|(zhì)結(jié)構(gòu)。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293T, 3: MCF-7, 4: A549
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 181 kDa
Observed MW: 200 kDa
Tissue: Mouse cerebrum
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60621R
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