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RPA2 Recombinant Rabbit mAb (bsm-60629R)  
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25ul/800.00元
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產(chǎn)品編號(hào) bsm-60629R
英文名稱 RPA2 Recombinant Rabbit mAb
中文名稱 復(fù)制因子A蛋白2重組兔單抗
別    名 RFA2_HUMAN; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2(RF-A protein 2); Replication protein A 34 kDa subunit(RP-A p34); REPA2; RPA32; RPA34; replication protein A2; RP-A p34;  
抗體來(lái)源 Rabbit
克隆類型 Recombinant
克 隆 號(hào) B10A1
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1:50-1:100,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 29 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 復(fù)制蛋白A(RPA)是一種保守的核單鏈 DNA (ssDNA)結(jié)合蛋白。人類RPA(hRPA)包含大約 70、32和14 kDa的三個(gè)亞基(hRPA70、hRPA32 和 hRPA14)。RPA32作為異源三聚體復(fù)制蛋白A復(fù)合物(RPA/RP-A)的一部分,結(jié)合并穩(wěn)定單鏈DNA中間體,這些中間體在DNA復(fù)制過(guò)程中形成。它可以防止它們重新退火,并同時(shí)招募和激活參與DNA代謝的不同蛋白質(zhì)和復(fù)合物。因此,它在DNA復(fù)制和細(xì)胞對(duì)DNA損傷的反應(yīng)中都起著至關(guān)重要的作用。
產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: MCF-7, 3: HepG2, 4: C6, 5: NIH-3T3 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 29 kDa Observed MW: 29 kDa
Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60629R
Tissue: Mouse small intestine Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60629R
Tissue: Rat liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60629R
Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for bsm-60629R
Cell line: NIH/3T3 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for bsm-60629R
Cell line: NIH/3T3 Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for bsm-60629R
Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for bsm-60629R
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