| 產(chǎn)品編號 | bsm-60016R |
| 英文名稱 | Rabbit Anti-MUC2 antibody |
| 中文名稱 | 粘蛋白-2/上皮膜抗原2重組兔單抗 |
| 別 名 | MLP; SMUC; Intestinal mucin 2; MUC 2; Mucin 2; Mucin 2 intestinal; Mucin 2 intestinal/tracheal; Mucin 2 oligomeric mucus/gel forming; Mucin 2 precursor; Mucin 2 tracheal; Mucin like protein; Mucin2; Intestinal mucin-2; MUC-2; Muc2; MUC2_HUMAN; Mucin-2; Mucin2. |
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Specific References (2) | bsm-60016R has been referenced in 2 publications.
[IF=5.988] Yang Liu. et al. Platycodon grandiflorus polysaccharides deeply participate in the anti-chronic bronchitis effects of platycodon grandiflorus decoction, a representative of "the lung and intestine are related". FRONT PHARMACOL. 2022 Sep 7;13:927384 IHC ; Rat.
[IF=3.829] Wang Ruibiao. et al. Effects of exosomes derived from Trichinella spiralis infective larvae on intestinal epithelial barrier function. VET RES. 2022 Dec;53(1):1-11 WB ; Pig.
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| 研究領域 | 腫瘤 細胞生物 免疫學 細胞粘附分子 糖蛋白 腫瘤細胞生物標志物 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 7F9 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 570kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MUC2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] Function: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Subunit: Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP. Interacts with AGR2; disulfide-linked. Subcellular Location: Secreted. Note=In the intestine, secreted into the inner and outer mucus layers. Tissue Specificity: Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. Post-translational modifications: O-glycosylated. May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. Similarity: Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 TIL (trypsin inhibitory-like) domain. Contains 2 VWFC domains. SWISS: Q02817 Gene ID: 4583 Database links: Entrez Gene: 4583 Human Entrez Gene: 17831 Mouse SwissProt: Q02817 Human SwissProt: Q80Z19 Mouse 細胞粘附蛋白（Call Adhesion Protein） 上皮膜抗原是粘性糖蛋白家族之一,是一種膜內在糖蛋白。Muc-2表達僅限于內膜腺上皮和腔上皮,很多上皮細胞及其來源的腫瘤表達該蛋白.其合成和分泌是腺上皮組織的特征之一。MUC-2也是一種主要的腸道粘蛋白，在正常腸道和結腸癌、胃癌等腫瘤中有廣泛分布。該抗體主要用于胃腸道腫瘤的研究。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Rat colon
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 540 kDa
Observed MW: 140-170, ＞250 kDa
Paraformaldehyde-fixed, paraffin embedded (Human duodenum ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (bsm-60016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (bsm-60016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (bsm-60016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60016R
Cell line: NIH/3T3
Fixative: 4% Paraformaldehyde
Permeabilization: 90% methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60016R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
