| 產(chǎn)品編號 | bsm-52824R |
| 英文名稱 | Rabbit Anti-NCAM antibody |
| 中文名稱 | 神經(jīng)細胞粘附分子1重組兔單抗 |
| 別 名 | CD56 antigen; Cell Adhesion Molecule Neural 1; MSK39; N CAM 120; N CAM 140; N CAM 180; NCAM 1; NCAM 120; NCAM 140; NCAM1; Neural cell adhesion molecule 1 120 kDa isoform; Neural cell adhesion molecule 1 140 kDa isoform; Neural cell adhesion molecule 1; NCAM1_HUMAN. |
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Specific References (1) | bsm-52824R has been referenced in 1 publications.
[IF=9.429] Zhang Yufan. et al. Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22 WB ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學(xué) 干細胞 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human,Mouse (predicted: Zebrafish) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 92kDa |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NCAM |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011] Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Subcellular Location : Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. Similarity: Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P13591 Gene ID: 4684 細胞粘附蛋白（Call Adhesion Protein） 神經(jīng)元標志物 NCAM-1為神經(jīng)細胞粘附分子，主要分布于神經(jīng)組織，神經(jīng)—肌肉接頭，神經(jīng)—內(nèi)分泌腺和某些內(nèi)分泌腺以及大多數(shù)神經(jīng)外胚層來源的細胞、組織和腫瘤中。 NCAM 1主要用于視網(wǎng)膜母細胞瘤、髓母細胞瘤、星形細胞瘤、神經(jīng)母細胞瘤等腫瘤方面的研究。CD56（神經(jīng)細胞黏附分子，NCAM）表達于大部分神經(jīng)外胚層來源的細胞系、組織和腫瘤如視網(wǎng)膜母細胞瘤、成神經(jīng)管細胞瘤、星形細胞瘤以及成神經(jīng)細胞瘤。它也表達于一些中胚層來源的腫瘤如橫紋肌肉瘤。NK細胞以及NK細胞淋巴瘤為陽性。CD56被認為是NK細胞腫瘤的重要標志物。該抗體識別分子量為180，145和125kDa亞型。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: SH-SY5Y, 2: Mouse cerebellum, 3: Rat cerebellum
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 95 kDa
Observed MW: 140-180 kDa
Western blot analysis of NCAM/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM/CD56 antibody (bsm-52824R) at 1/500 dilution.
Lysates/proteins at 10 μg/Lane.
Predicted band size: 95 kDa
Observed band size: 120/140 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Western blot analysis of NCAM/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody (bsm-52824R) at 1/2,000 dilution.
Lysates/proteins at 20 μg/Lane.
Predicted band size: 95 kDa
Observed band size: 140/180 kDa
Exposure time: 30 seconds;
6% SDS-PAGE gel.
Paraformaldehyde-fixed, paraffin embedded (human Prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM) Monoclonal Antibody, Unconjugated (bsm-52824R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NCAM
) monoclonal Antibody, Unconjugated (bsm-52824R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
