| 產(chǎn)品編號 | bs-23586R |
| 英文名稱 | GDNF Rabbit pAb |
| 中文名稱 | 膠質(zhì)細胞源性神經(jīng)營養(yǎng)因子抗體 |
| 別 名 | glial cell line derived neurotrophic factor; Astrocyte derived trophic factor 1; Astrocyte derived trophic factor; ATF 1; ATF 2; ATF1; ATF2; Glial cell derived neurotrophic factor; Glial derived neurotrophic factor; HFB1 GDNF; GDNF_HUMAN; Atf; gdnf; Glial |
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Specific References (3) | bs-23586R has been referenced in 3 publications.
[IF=4.285] Xi Huaming. et al. Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season. Biol Reprod. 2021 Aug;: WB ; Goat.
[IF=4.102] Huaming Xi. et al. FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells. Mol Cell Endocrinol. 2021 Nov;:111505 WB ; Goat.
[IF=2.923] Huaming Xi. et al. FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway. THERIOGENOLOGY. 2023 Jan;195:103 WB, IF ; Goat.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 細胞凋亡 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDNF : 151-211/211 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Neurobiology. Neurotrophins. Neuroscience. This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted. Tissue Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3) [MIM:613711]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Similarity: Belongs to the TGF-beta family. GDNF subfamily. SWISS: P39905 Gene ID: 2668 Database links: Entrez Gene: 2668 Human Entrez Gene: 14573 Mouse Omim: 600837 Human SwissProt: P39905 Human SwissProt: P48540 Mouse Unigene: 248114 Human Unigene: 4679 Mouse Unigene: 53970 Rat |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-GDNF ?(bs-23586R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 20 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-GDNF (bs-23586R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 19 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GDNF ?(bs-23586R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (bs-23586R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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