| 產品編號 | bsm-33157M |
| 英文名稱 | Mouse Anti-HA Tag antibody |
| 中文名稱 | HA tag（標簽）單克隆抗體 |
| 別 名 | HA-tag; HA epitope tag; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2. |
| 產品類型 | 標簽抗體 |
| 研究領域 | 細胞生物 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 4D1 |
| 交叉反應 | Recombinant protein |
| 產品應用 | WB=1:50000-1:100000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated HA Tag |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. SWISS: N/A Gene ID: N/A |
| 產品圖片 |
Sample: Lane1: HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Lane2: HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Primary: Anti-HA-Tag (bsm-33157M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
Sample:
HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug
Primary: Anti-HA-Tag (bsm-33157M) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Sample:
HA-Tagged fusion protein
Primary: Anti-HA-tag/HRP (bsm-33157M-HRP) at 1/100000 dilution
Observed band size: 42 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
