| 產品編號 | bs-12777R |
| 英文名稱 | Rabbit Anti-VPS45A antibody |
| 中文名稱 | 液泡蛋白分選蛋白45A抗體 |
| 別 名 | h-VPS45; H1; H1VPS45; HGNC:14579; hlVps45; Leucocyte vacuolar protein sorting 45; OTTHUMP00000014482; Vacuolar protein sorting 45 homolog (S. cerevisiae); Vacuolar protein sorting 45A (yeast homolog); Vacuolar protein sorting 45A; Vacuolar protein sorting 45B (yeast); Vacuolar protein sorting-associated protein 45; VPS45; VPS45_HUMAN; VPS45A; VPS45B; VPS54A; VSP45; VSP45A. |
| 研究領域 | 細胞生物 信號轉導 跨膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VPS45A: 401-500/570 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013] Function: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. Subcellular Location: Golgi apparatus membrane. Endosome membrane. Associated with Golgi/endosomal vesicles and the trans-Golgi network. Tissue Specificity: Ubiquitous. Expression was highest in testis, heart and brain, intermediate in kidney, spleen, prostate, ovary, small intestine and thymus and low in lung, skeletal muscle, placenta, colon, pancreas, peripheral blood leukocytes and liver. Similarity: Belongs to the STXBP/unc-18/SEC1 family. SWISS: Q9NRW7 Gene ID: 11311 Database links: Entrez Gene: 430741 Chicken Entrez Gene: 11311 Human Entrez Gene: 22365 Mouse Omim: 610035 Human SwissProt: Q9NRW7 Human SwissProt: P97390 Mouse Unigene: 443750 Human Unigene: 263185 Mouse Unigene: 9316 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VPS45A) Polyclonal Antibody, Unconjugated (bs-12777R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
