| 產(chǎn)品編號 | bs-19998R |
| 英文名稱 | PACSIN2 Rabbit pAb |
| 中文名稱 | 胞漿磷蛋白PACSIN2抗體 |
| 別 名 | cytoplasmic phosphoprotein PACSIN2; Moderately similar to h74 gene product; OTTHUMP00000198281; OTTHUMP00000198282; OTTHUMP00000198283; OTTHUMP00000198284; PACN2_HUMAN; Pacsin2; protein kinase C and casein kinase substrate in neurons 2; protein kinase C and casein kinase substrate in neurons 2; Protein kinase C and casein kinase substrate in neurons protein 2; Protein kinase C and casein kinase substrate in neurons protein; SDP II; SDPII; Syndapin II. |
| 研究領域 | 細胞生物 神經(jīng)生物學 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PACSIN2: 331-430/486 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] Function: May play a role in vesicle formation and transport. Subunit: Homodimer. May form heterooligomers with other PACSINs.Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4. Subcellular Location: Cytoplasmic vesicle. Vesicle-like cytoplasmic distribution. Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). Similarity: Belongs to the PACSIN family. Contains 1 FCH domain. Contains 1 SH3 domain. SWISS: Q9UNF0 Gene ID: 11252 Database links: Entrez Gene: 11252 Human Entrez Gene: 23970 Mouse Omim: 604960 Human SwissProt: Q9UNF0 Human SwissProt: Q9WVE8 Mouse Unigene: 162877 Human Unigene: 23978 Mouse |
| 產(chǎn)品圖片 |
Sample: Lung (mouse) Lysate at 40 ug
Primary: Anti-PACSIN2 (bs-19998R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kD
Observed band size: 56kD
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PACSIN2) Polyclonal Antibody, Unconjugated (bs-19998R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PACSIN2) Polyclonal Antibody, Unconjugated (bs-19998R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
