| 產(chǎn)品編號 | bs-14142R |
| 英文名稱 | Rabbit Anti-CYLC2 antibody |
| 中文名稱 | CYLC2蛋白抗體 |
| 別 名 | CYL2; CYLC 2; Cylicin 2; Cylicin basic protein of sperm head cytoskeleton 2; Cylicin II; Cylicin2; Cylicin-2; MGC129591; Multiple band polypeptide II; CYLC2_HUMAN. |
| 研究領域 | 細胞生物 發(fā)育生物學 干細胞 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYLC2: 21-120/348 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cylicin-2 is a 348 amino acid protein encoded by the human gene CYLC2. Cylicin-1 is a type of a cytoskeletal protein which contains numerous Lys-Lys-Asp tripeptides accumulated in nine central repetitive units predicted to form a-helices. Cylicin-2, present in cow and human sperm heads, has the same prominent molecular characteristics as Cylicin-1, including a high content of charged amino acids, the abundance of Lys-Lys-Asp tripeptides and repetitive units of presumably a-helical configuration. Cylicin-2 is found in the acrosomal region of round spermatids and in the post-acrosomal region of late spermatids and spermatozoa, in agreement with the localization of Cylicin-1. Cylicin-2 is a novel Actin-binding protein, which probably plays a role in the Actin-related events that occur during spermiogenesis and the early events of fertilization. Function: CYCL2 (Cylicin II) is specifically expressed in testis and is part of the cytoskeletal calyx of mammalian sperm heads. Cylicin II may play a role in the morphogenesis of the sperm head and may be involved in spermatid differentiation. Subcellular Location: Cytoplasm, cytoskeleton, perinuclear theca, calyx. Note=Sperm head cytoskeletal structure. Tissue Specificity: Testis. SWISS: Q14093 Gene ID: 1539 Database links: Entrez Gene: 1539 Human SwissProt: Q14093 Human |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
