| 產(chǎn)品編號 | bs-13713R |
| 英文名稱 | Rabbit Anti-AJAP1 antibody |
| 中文名稱 | 粘合連接相關(guān)蛋白1抗體 |
| 別 名 | Adherens junction-associated protein 1; Adherens junction-associated protein 1; Ajap1; AJAP1_HUMAN; Membrane protein shrew-1; MOT8; SHREW-1; SHREW1 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞粘附分子 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AJAP1: 341-411/411 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Plays a role in cell adhesion and cell migration. Subcellular Location: Basolateral cell membrane. Apical cell membrane. Cell junction > adherens junction. Mainly basolateral. Localization is mediated by AP1M2. Tissue Specificity: Expressed in uterus and pancreas (at protein level). Post-translational modifications: Thr-237 and Ser-239 may be phosphorylated; however as this position is probably extracellular, the in vivo relevance is not proven. SWISS: Q9UKB5 Gene ID: 55966 Database links: Entrez Gene: 55966 Human SwissProt: Q9UKB5 Human Unigene: 25924 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Uterus (Mouse) Lysate at 40 ug
Lane 6: Uterus (Rat) Lysate at 40 ug
Lane 7: Pancreas (Mouse) Lysate at 40 ug
Lane 8: Pancreas (Rat) Lysate at 40 ug
Lane 9: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-AJAP1 (bs-13713R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
