| 產(chǎn)品編號 | bs-11018R |
| 英文名稱 | Rabbit Anti-GALNT14 antibody |
| 中文名稱 | GalNAc-T14抗體 |
| 別 名 | GalNAc T14; GalNAc-T14; Galnt14; GALNT14; GalNac-T10; GALNT15; pp-GaNTase 14; GLT14_HUMAN; Polypeptide GalNAc transferase 14; Polypeptide N acetylgalactosaminyltransferase 14; Polypeptide N-acetylgalactosaminyltransferase 14; pp GaNTase 14; Protein UDP acetylgalactosaminyltransferase 14; Protein-UDP acetylgalactosaminyltransferase 14; UDP GalNAc:polypeptide N acetylgalactosaminyltransferase 14; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 14. |
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Specific References (2) | bs-11018R has been referenced in 2 publications.
[IF=3.071] Shan et al. GALNT14 Involves the Regulation of Multidrug Resistance in Breast Cancer Cells. (2018) Transl.Oncol. 11:786-793 IHC ; Human.
[IF=2.33] Yang, Juan, Guiyuan Li, and Keqiang Zhang. "Pro-survival effects by NF-κB, Akt and ERK (1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer." Biomedicine & Pharmacotherapy 84 (2016): 1078-1087. WB ; Human.
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| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 通道蛋白 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GALNT14: 101-200/552 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a Golgi protein which is a member of the polypeptide N-acetylgalactosaminyltransferase (ppGalNAc-Ts) protein family. These enzymes catalyze the transfer of N-acetyl-D-galactosamine (GalNAc) to the hydroxyl groups on serines and threonines in target peptides. The encoded protein has been shown to transfer GalNAc to large proteins like mucins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]. Function: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays activity toward mucin-derived peptide substrates such as Muc2, Muc5AC, Muc7, and Muc13 (-58). May be involved in O-glycosylation in kidney. Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Tissue Specificity: Highly expressed in fetal and adult kidney. Widely expressed at low level. Weakly expressed in whole brain, cerebellum, thymus, lung, mammary gland, liver, stomach, small intestine, colon, pancreas, spleen, bladder, uterus, placenta, testis, ovary, skeletal muscle, leukocyte, B-cell, bone marrow, fetal brain, fetal thymus, fetal lung, fetal liver, fetal small intestine, fetal spleen, fetal skeletal and fetus. Similarity: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain. SWISS: Q96FL9 Gene ID: 79623 Database links: Entrez Gene: 79623 Human Entrez Gene: 71685 Mouse Omim: 608225 Human SwissProt: Q96FL9 Human SwissProt: Q8BVG5 Mouse Unigene: 468058 Human Unigene: 271953 Mouse Unigene: 24642 Rat |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
MG63(Human) Cell Lysate at 30 ug
Primary: Anti- GALNT14 (bs-11018R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 66 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
