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Mouse Anti-CEA  antibody (bsm-4792M)  
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產(chǎn)品編號 bsm-4792M
英文名稱 Mouse Anti-CEA  antibody
中文名稱 癌胚抗原單克隆抗體
別    名 Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100.  
Specific References  (3)     |     bsm-4792M has been referenced in 3 publications.
[IF=5.83] Ren, Kewei, et al. "Target-Driven Triple-Binder Assembly of MNAzyme for Amplified Electrochemical Immunosensing of Protein Biomarker." Analytical Chemistry (2015).  other ;  
[IF=5.123] Cao Y et al. Based on ZnSe quantum dots labeling and single particle mode ICP-MS coupled with sandwich magnetic immunoassay for the detection of carcinoembryonic antigen in human serum.Anal Chim Acta. 2018 Oct 22;1028:22-31.  Other ;  Human.  
[IF=4.244] Xu?J et al. Surface plasmon resonance sensing with adjustable sensitivity based on a flexible liquid core coupling unit. Talanta. 2018 Jul 1;184:468-474.  other ;  Human.  
研究領(lǐng)域 腫瘤  免疫學(xué)  干細(xì)胞  腫瘤細(xì)胞生物標(biāo)志物  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 4F3
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CEA/CD66e/CEACAM5: 41-140/702 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Function:
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.

Subunit:
Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor

Tissue Specificity:
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Similarity:
Belongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains.

SWISS:
P06731

Gene ID:
1048

Database links:

Entrez Gene: 1048 Human

Omim: 114890 Human

SwissProt: P06731 Human

Unigene: 709196 Human



CEA是一種胚胎性抗原,主要存在于胎兒消化道上皮組織,胰腺和肝癌中。CEA廣泛存在各種上皮性腫瘤,尤其是胃腸道惡性腫瘤,CEA分布陽性類型與腫瘤的惡性度有關(guān)。CEA與EMA一樣可作為上皮性惡性腫瘤的重要標(biāo)記。
產(chǎn)品圖片
Sample: Lane 1: Human LOVO cell lysates Lane 2: Recombinant human CEACAM5 protein, C-His Primary: Anti-CEACAM5 (bsm-4792M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 77 kDa Observed band size: 200,140 kDa
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA) Monoclonal Antibody, Unconjugated (bsm-4792M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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