| 產(chǎn)品編號 | bs-7410R |
| 英文名稱 | Rabbit Anti-ATP6V0A1 antibody |
| 中文名稱 | 氫離子轉(zhuǎn)運ATP合成酶A1抗體 |
| 別 名 | V ATPase; V ATPase A; V ATPase A1; Vacuolar proton ATPase a1; V-type proton ATPase 95 kDa subunit a isoform 1; V-ATPase 95 kDa isoform a1; AltName: Full=Vacuolar proton pump subunit a1; Vacuolar proton translocating ATPase 95 kDa subunit a isoform 1; VPP1_HUMAN. |
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Specific References (1) | bs-7410R has been referenced in 1 publications.
[IF=7.088] Xinyi Qi. et al. Briarane-type diterpenoids, the inhibitors of osteoclast formation by interrupting Keap1-Nrf2 interaction and activating Nrf2 pathway. EUR J MED CHEM. 2023 Jan;246:114948 WB ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 激酶和磷酸酶 通道蛋白 細胞膜受體 轉(zhuǎn)運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 96kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human V-ATPase A1: 41-140/837 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The subunit of the vacuolar proton pump is a V-ATPase that has two different isoforms. The type I isoform contains an 18-base pair insert and is expressed in brain, whereas the truncated type II isoform is more widely expressed, including lung, kidney and spleen. The subunit of the vacuolar proton pump is located in clathrin-coated vesicles and is also found in osteoclasts. It consists of two fundamental domains, a hydrophilic amino-terminus, which has greater than 30% charged residues, and a hydrophobic carboxy terminus, which contains at least six transmembrane regions. The proton pump functions in coupling ATP hydrolysis by the cytoplasmic subunits to proton translocation by the intramembranous components of the pump. The inactivation of the osteoclast-specific vacuolar proton ATPase subunit is responsible for the lack of the enzyme in the apical membranes of osteoclast cells in osteosclerotic mutant mice, thus preventing the resorption function of these cells and leading to the osteopetrotic phenotype. The subunit, which co-localizes with the late endosomal marker Rab7 on vacuolar membranes, is essential for vacuole formation by selective swelling of late endosomes. Function: Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity). Subunit: The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''. Subcellular Location: Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note=Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Similarity: Belongs to the V-ATPase 116 kDa subunit family. SWISS: Q93050 Gene ID: 535 Database links: Entrez Gene: 395474 Chicken Entrez Gene: 535 Human Entrez Gene: 11975 Mouse Omim: 192130 Human SwissProt: Q9I8D0 Chicken SwissProt: Q93050 Human SwissProt: Q9Z1G4 Mouse Unigene: 463074 Human Unigene: 340818 Mouse Unigene: 475829 Mouse Unigene: 6015 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-V-ATPase A1 Polyclonal Antibody, Unconjugated(bs-7410R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
