| 產(chǎn)品編號 | bs-11643R |
| 英文名稱 | Rabbit Anti-CLACP antibody |
| 中文名稱 | 阿爾茨海默病淀粉樣蛋白相關(guān)膠原蛋白抗體 |
| 別 名 | Alzheimer disease amyloid associated protein; AMY; CLAC; CLAC P; CLAC precursor; COL25A1; COL25A1 protein; Collagen alpha 1 XXV chain; Collagen alpha 1(XXV) chain; Collagen like Alzheimer amyloid plaque component; Collagen like Alzheimer amyloid plaque component precursor; Collagen type XXV alpha 1; Collagenous Alzheimer amyloid plaque component. |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CLACP: 581-654/654 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Collagen proteins (products of the COL gene family) are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. The Collagen Type XXV precursor produces the proteolytic fragment CLAC (collagenous Alzheimer amyloid plaque component), which deposits in senile plaques associated with amyloid beta peptides (Abeta) in the brains of patients with Alzheimer's disease. CLAC binds to the fibrillized form of Abeta, which disturbs the structure and function of plasma membranes. Function: Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100 kDa polypeptides in Alzheimer's diseased (AD) brains. The 9D2 antigen was identified as CLAC (collagen like Alzheimer amyloid plaque component), and its precursor as CLACP/collagen type XXV. Both secreted and membrane tethered forms of CLACP/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta amyloidogenesis and neuronal degeneration in AD. Subunit: o assemble amyloid fibrils into protease-resistant aggregates. Binds heparin. [SUBUNIT] Forms homodimers and homotrimers. Binds to the fibrillized forms of beta amyloid peptide 40 (beta-APP40) and beta amyloid peptide 42 (beta-APP42). Found associated with beta-APP42 more frequently than with beta-APP40. Subcellular Location: Membrane; Single-pass type II membrane protein (Potential). Note=After proteolytic cleavage, CLAC is secreted. Tissue Specificity: Expressed predominantly in brain. Deposited preferentially in primitive or neuritic amyloid plaques which are typical of Alzheimer disease. Post-translational modifications: Undergoes proteolytic cleavage by furin protease to yield the soluble collagen-like Alzheimer amyloid plaque component. Glycosylated. Hydroxylated on 11% of proline residues and 49% of lysine residues. Similarity: Contains 7 collagen-like domains. SWISS: Q9BXS0 Gene ID: 84570 Database links: Entrez Gene: 84570 Human Entrez Gene: 77018 Mouse Omim: 610004 Human SwissProt: Q9BXS0 Human SwissProt: Q99MQ5 Mouse Unigene: 658842 Human Unigene: 91728 Mouse |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- CLACP (bs-11643R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 57 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CLACP Polyclonal Antibody, Unconjugated(bs-11643R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLACP) Polyclonal Antibody, Unconjugated (bs-11643R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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