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Rabbit Anti-RBM20  antibody (bs-9606R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-9606R
英文名稱 Rabbit Anti-RBM20  antibody
中文名稱 RNA結合蛋白20抗體
別    名 Probable RNA-binding protein 20; Rbm20; RBM20_HUMAN; RNA-binding motif protein 20.  
研究領域 心血管  轉錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Sheep,Cow,Dog,Horse)
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 134kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RBM20: 361-460/1227 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein that likely binds RNA. Mutations in this gene have been associated with familial dilated cardiomyopathy. [provided by RefSeq, Mar 2010]

Function:
RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin).

Subcellular Location:
Nucleus (By similarity).

Tissue Specificity:
Expressed in the heart.

DISEASE:
Defects in RBM20 are the cause of cardiomyopathy dilated type 1DD (CMD1DD) [MIM:613172]. A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
Q5T481

Gene ID:
282996

Database links:

Entrez Gene: 282996 Human

Entrez Gene: 73713 Mouse

Entrez Gene: 309544 Rat

Omim: 613171 Human

SwissProt: Q5T481 Human

SwissProt: Q3UQS8 Mouse

Unigene: 92105 Cow

Unigene: 116630 Human

Unigene: 726550 Human

Unigene: 485879 Mouse

Unigene: 21596 Rat



Involvement in disease;Defects in RBM20 are the cause of cardiomyopathy dilated type 1DD (CMD1DD). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
產(chǎn)品圖片
Sample: heart (mouse) Lysate at 40 ug Primary: Anti- RBM20 (bs-9606R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75kD Observed band size: 130 kD
Sample: K562(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- RBM20 (bs-9606R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kD Observed band size: 130 kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RBM20 Polyclonal Antibody, Unconjugated(bs-9606R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:A549. Primary Antibody (green line): Rabbit Anti-RBM20 antibody (bs-9606R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(black line):A549. Primary Antibody (green line): Rabbit Anti-RBM20 antibody (bs-9606R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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