| 產(chǎn)品編號 | bs-7798R |
| 英文名稱 | Rabbit Anti-Flotillin 1 antibody |
| 中文名稱 | 脂閥結(jié)構(gòu)蛋白1抗體 |
| 別 名 | FLOT 1; FLOT1; FLOT1_HUMAN; Flotillin-1; Flotillin1; Integral membrane component of caveolae; Reggie 2. |
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Specific References (2) | bs-7798R has been referenced in 2 publications.
[IF=3.989] Capomaccio S et al. Equine Adipose-Derived Mesenchymal Stromal Cells Release Extracellular Vesicles Enclosing Different Subsets of Small RNAs.Stem Cells Int. 2019 Mar 18;2019:4957806. Immunoelectron Microscopy ; Human.
[IF=3.23] Matsuzaka, Yasunari, et al. "Characterization and Functional Analysis of Extracellular Vesicles and Muscle-Abundant miRNAs (miR-1, miR-133a, and miR-206) in C2C12 Myocytes and mdx Mice." PLOS ONE 11.12 (2016): e0167811. IP ; Human, Mouse.
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| 研究領(lǐng)域 | 細胞生物 免疫學 信號轉(zhuǎn)導 細胞周期蛋白 細胞骨架 細胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Flotillin 1: 101-200/427 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Function: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Subunit: Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECM29. Subcellular Location: Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Melanosome. Endosome. Similarity: Belongs to the band 7/mec-2 family. Flotillin subfamily. SWISS: O75955 Gene ID: 10211 Database links: Entrez Gene: 10211 Human Entrez Gene: 14251 Mouse Omim: 606998 Human SwissProt: O75955 Human SwissProt: O08917 Mouse Unigene: 179986 Human |
| 產(chǎn)品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-Flotillin 1 (bs-7798R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Primary: Anti-Flotillin 1 (bs-7798R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
