| 產(chǎn)品編號 | bs-7535R |
| 英文名稱 | Rabbit Anti-PGC1 alpha + beta antibody |
| 中文名稱 | 過氧化物酶體增殖物激活受體γ輔激活子1α+β抗體 |
| 別 名 | LEM6; Ligand effect modulator 6; PERC; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; peroxisome proliferator-activated receptor gamma coactivator 1 beta; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-beta; peroxisome proliferator-activated receptor gamma, coactivator 1 beta; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC-1(beta); PGC-1-alpha; PGC-1-beta; PGC-1-related estrogen receptor alpha coactivator; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1beta; PPAR-gamma coactivator 1-alpha; PPAR-gamma coactivator 1-beta; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC-1-beta; PPARGC1; PPARGC1A; Ppargc1b; PRGC1_HUMAN; PRGC2_HUMAN. |
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Specific References (6) | bs-7535R has been referenced in 6 publications.
[IF=5.279] Lei Wang. et al. Lactobacillus plantarum DP189 Reduces α-SYN Aggravation in MPTP-Induced Parkinson’s Disease Mice via Regulating Oxidative Damage, Inflammation, and Gut Microbiota Disorder. J Agr Food Chem. 2022;70(4):1163–1173 WB ; Mouse.
[IF=5.26] Meng, Guoliang, et al. "Hydrogen sulfide pretreatment improves mitochondrial function in myocardial hypertrophy via a SIRT3 dependent manner." British Journal of Pharmacology (2017). WB ; Mouse.
[IF=4.315] Ma, Dufang. et al. Astragalus polysaccharide prevents heart failure-induced cachexia by alleviating excessive adipose expenditure in white and brown adipose tissue. LIPIDS HEALTH DIS. 2023 Dec;22(1):1-16 WB ; Rat.
[IF=3.776] Tingting Zhao. et al. Yishen Huashi granule modulated lipid metabolism in diabetic nephropathy via PI3K/AKT/mTOR signaling pathways.. HELIYON. 2023 Mar;9(3):e14171-e14171 WB ; Human.
[IF=3.189] Tingting Li. et al. Cold exposure induces browning of bovine subcutaneous white fat in vivo and in vitro. J THERM BIOL. 2023 Feb;112:103446 WB ; Bovine.
[IF=2.952] Ji Hye Hwang. et al. Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway. MOL MED REP. Mol Med Rep. 2022 Jun;25(6):1-10 WB ; Mouse.
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| 研究領(lǐng)域 | 心血管 細胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 糖尿病 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat,Pig (predicted: Mouse,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 88/113kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PGC1 alpha + beta: 151-250/798 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq]. Function: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Subunit: Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with PRDM16. Interacts with LRPPRC. Homooligomer. Interacts with LPIN1. Subcellular Location: Nucleus. Tissue Specificity: Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. Post-translational modifications: Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q9UBK2 Gene ID: 10891 Database links: Entrez Gene: 10891 Human Entrez Gene: 133522 Human Entrez Gene: 170826 Mouse Entrez Gene: 19017 Mouse Omim: 604517 Human Omim: 608886 Human SwissProt: Q86YN6 Human SwissProt: Q9UBK2 Human SwissProt: 415302 Mouse SwissProt: O70343 Mouse SwissProt: Q8VHJ7 Mouse Unigene: 483816 Human Unigene: 527078 Human Unigene: 163382 Rat |
| 產(chǎn)品圖片 |
Sample:
Muscle (Pig) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-PGC1 alpha + beta (bs-4064R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 88/113 kD
Observed band size: 92 kD
Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88/113 kD
Observed band size: 113 kD
Sample:
Lane 1: Molt-4 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: HL60 (Human) Cell Lysate at 30 ug
Primary: Anti-PGC1 alpha + beta (bs-7535R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90/113 kD
Observed band size: 113 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGC1 alpha + beta) Polyclonal Antibody, Unconjugated (bs-7535R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PGC1 alpha+beta Polyclonal Antibody, Unconjugated(bs-7535R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-PGC1 alpha+beta antibody (bs-7535R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
