| 產(chǎn)品編號 | bs-6663R |
| 英文名稱 | Rabbit Anti-HRH1 antibody |
| 中文名稱 | 組胺受體H1抗體 |
| 別 名 | Histamine H1 receptor; H1R; HisH1; Histamine receptor H1; Histamine receptor subclass H1; HRH1_HUMAN. |
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Specific References (6) | bs-6663R has been referenced in 6 publications.
[IF=11.685] Kristine Elisabeth Eberhard. et al. Neurotransmitter and Neurotransmitter Receptor Expression in the Saccule of the Human Vestibular System. Prog Neurobiol. 2022 Jan;:102238 IHC ; Human.
[IF=6.038] Liao, Xiaodan. et al. Fullerene nanoparticles for the treatment of ulcerative colitis. 2021 Nov 02 WB ; Rat.
[IF=3.263] Aya Sato. et al. Histamine depolarizes rat intracardiac ganglion neurons through the activation of TRPC non-selective cation channels. Eur J Pharmacol. 2020 Nov;886:173536 WB ; Rat.
[IF=2.629] Yu Wen-Yan. et al. Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation. Evid-Based Compl Alt. 2022;2022:4571981 WB ; Rat.
[IF=1.54] M?ller, M. Nue, et al. "Expression of histamine receptors in the human endolymphatic sac: the molecular rationale for betahistine use in Menieres disease." European Archives of Oto-Rhino-Laryngology: 1-6. IHC-P ; Human.
[IF=1.504] Maheswari DU et al. Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo. South African Journal of Botany, 2020, 130, 130–140. WB ; Mouse.
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| 研究領域 | 細胞生物 免疫學 信號轉導 生長因子和激素 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HRH1: 141-240/487 <Extracellular> |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]. Function: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P35367 Gene ID: 3269 Database links: Entrez Gene: 3269 Human Entrez Gene: 15465 Mouse Omim: 600167 Human SwissProt: P35367 Human SwissProt: P70174 Mouse Unigene: 1570 Human Unigene: 333327 Mouse Unigene: 177519 Rat Unigene: 81032 Rat |
| 產(chǎn)品圖片 |
Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-HRH1 (bs-6663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-HRH1 (bs-6663R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HRH1) Polyclonal Antibody, Unconjugated (bs-6663R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
