| 產品編號 | bs-4145R |
| 英文名稱 | SYTL5 Rabbit pAb |
| 中文名稱 | 突觸樣蛋白5亞型2抗體 |
| 別 名 | synaptotagmin-like protein 5 isoform 2; synaptotagmin-like protein 5; SYTL5 protein; synaptotagmin-like 5; ENSMUSG00000054453; slp5; SYTL5. |
| 研究領域 | 免疫學 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 81 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SYTL5: 601-700/730 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene belongs to the synaptotagmin-like (Slp) protein family, which contains a unique homology domain at the N-terminus, referred to as the Slp homology domain (SHD). The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of this gene is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Function: May act as Rab effector protein and play a role in vesicle trafficking. Binds phospholipids. Subcellular Location: Membrane. Tissue Specificity: Highly expressed in placenta and liver. Similarity: Contains 2 C2 domains. Contains 1 FYVE-type zinc finger. Contains 1 RabBD (Rab-binding) domain. SWISS: Q8TDW5 Gene ID: 94122 Database links: Entrez Gene: 94122 Human Entrez Gene: 236643 Mouse SwissProt: Q8TDW5 Human SwissProt: Q80T23 Mouse Unigene: 662334 Human Unigene: 246606 Mouse Unigene: 92480 Rat |
| 產品圖片 |
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human A549 cell lysates
Primary: Anti-SYTL5 (bs-4145R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SYTL5 Polyclonal Antibody, Unconjugated(bs-4145R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
