| 產(chǎn)品編號 | bs-5384R |
| 英文名稱 | Rabbit Anti-phospho-HMG14 (Ser20+Ser24) antibody |
| 中文名稱 | 磷酸化高遷移率核小體結(jié)合蛋白1 |
| 別 名 | HMG14 (phospho Ser20+Ser24); HMG14 (phospho S20 + S24); High mobility group (nonhistone chromosomal) protein 14; High mobility group nucleosome binding 1; High mobility group nucleosome binding domain containing protein 1; High mobility group protein 14; High-mobility group nucleosome binding domain 1; HMG14; HMGN 1; HMGN1; MGC104230; MGC117425; Nonhistone chromosomal protein HMG-14; Nonhistone chromosomal protein HMG14; FLJ27265; FLJ31471. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 染色質(zhì)和核信號 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 結(jié)合蛋白 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human HMG14 around the phosphorylation site of Ser20+Ser24: RR(p-S)ARL(p-S)AK |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Chromosomal protein HMG14 binds to the inner side of the nucleosomal DNA, thus altering the interaction between the DNA and the histone octamer and is involved in the process that maintains transcribable genes in an appropriate chromatin conformation. HMG14 has been implicated as a contributing factor in the etiology of Down syndrome. Function: Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2. Subcellular Location: Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus. Post-translational modifications: Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization. Similarity: Belongs to the HMGN family. SWISS: P05114 Gene ID: 3150 Database links: Entrez Gene: 3150 Human Entrez Gene: 100044391 Mouse Entrez Gene: 15312 Mouse Omim: 163920 Human SwissProt: P05114 Human SwissProt: P18608 Mouse Unigene: 87121 Cow Unigene: 356285 Human Unigene: 702472 Human Unigene: 2756 Mouse HMGN1是一個核仁結(jié)合蛋白, 屬于核小體結(jié)合高速遷移蛋白家族(A family of High Mobility Group Proteins that bind to Nucleosomesn, HMGN)含量蛋白較高,能穩(wěn)定染色質(zhì)的二級結(jié)構(gòu), 修復(fù)紫外線誘導(dǎo)的染色質(zhì)DNA損傷. HMGN1的缺失可導(dǎo)致細(xì)胞增殖和生長發(fā)生紊亂, 通過打亂細(xì)胞的生長控制機(jī)制使一些致癌因素更為敏感并發(fā)生惡性轉(zhuǎn)化. 研究表明, HMGN1在細(xì)胞核內(nèi)是動態(tài)表達(dá)的,與細(xì)胞的新陳代謝有關(guān). HMGN1蛋白和核仁的相互作用可改變?nèi)旧|(zhì)的結(jié)構(gòu), 并且能調(diào)節(jié)各種與DNA相關(guān)的包括轉(zhuǎn)錄的細(xì)胞核作用. |
