| 產(chǎn)品編號(hào) | bs-2377R |
| 英文名稱 | CNR2 Rabbit pAb |
| 中文名稱 | 鈣粘蛋白相關(guān)的神經(jīng)受體2抗體 |
| 別 名 | Cannabinoid receptor 2; Cannabinoid Receptor II; Cannabinoid receptor 2 macrophage; CB 2; CB-2; CB2; CNR2; CB2R; CNRII; CNR-2; CNR 2; CX 5; CX5; CNR2_HUMAN. |
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Specific References (4) | bs-2377R has been referenced in 4 publications.
[IF=6.208] Uliana De Simone. et al. Human Astrocyte Spheroids as Suitable In Vitro Screening Model to Evaluate Synthetic Cannabinoid MAM2201-Induced Effects on CNS. INT J MOL SCI. 2023 Jan;24(2):1421 IF,FCM ; Human.
[IF=5.94] Braun, Molly, et al. "Selective activation of cannabinoid receptor-2 reduces neuroinflammation after traumatic brain injury via alternative macrophage polarization." Brain, Behavior, and Immunity (2017). IHC-P ; Mouse.
[IF=3.022] Xu A et al. Inhibiting effect of microRNA-187-3p on osteogenic differentiation of osteoblast precursor cells by suppressing cannabinoid receptor type 2. Differentiation. 2019 Jul 13;109:9-15. ICF ; Human.
[IF=0.68] Pihlaja R et al. Long-Term Monoacylglycerol Lipase Inhibitor Treatment Decelerates Pathological Changes in APP/PS1-21 Mice, but Behavioral Improvements Require Early-Stage Treatment Onset—Short Report. World Journal of Neuroscience, 2018, 8, 157-170. IHC ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CNR2: 251-350/360 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]. Function: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. Subcellular Location: Cell membrane. Cell projection, dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons. Tissue Specificity: Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level). Post-translational modifications: Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P34972 Gene ID: 1269 Database links: Entrez Gene: 1269 Human Entrez Gene: 12802 Mouse Omim: 605051 Human SwissProt: P34972 Human SwissProt: P47936 Mouse Unigene: 73037 Human Unigene: 297251 Mouse Unigene: 48776 Rat 大麻素受體2(大麻素II型受體)CB2受體在正常皮膚主要分布于上皮細(xì)胞、巨噬細(xì)胞、肥大細(xì)胞、感覺神經(jīng)纖維、毛囊、汗腺等部位, 參與皮膚感覺傳導(dǎo)、疼痛機(jī)制、皮膚免疫、皮膚腫瘤、細(xì)胞分化等重要的生理病理過程. |
| 產(chǎn)品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CNR2/CB2 Polyclonal Antibody, Unconjugated(bs-2377R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNR2) Polyclonal Antibody, Unconjugated (bs-2377R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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