| 產(chǎn)品編號 | bs-4064R |
| 英文名稱 | Rabbit Anti-IGFBP6 antibody |
| 中文名稱 | 胰島素樣生長因子結合蛋白6抗體 |
| 別 名 | IBP 6; IBP-6; IBP6; IBP6_HUMAN; IGF binding protein 6; IGF-binding protein 6; IGFBP 6; IGFBP-6; Insulin like growth factor binding protein 6; Insulin-like growth factor-binding protein 6. |
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Specific References (2) | bs-4064R has been referenced in 2 publications.
[IF=4.2] Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829. WB ; Mouse.
[IF=2.123] Liqiang Zhou. et al. Tectorigenin alleviates the apoptosis and inflammation in spinal cord injury cell model through inhibiting insulin-like growth factor-binding protein 6. OPEN MED-WARSAW. 2023 Jan;18(1): WB ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 生長因子和激素 轉錄調(diào)節(jié)因子 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IGFBP6: 141-240/240 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Subcellular Location: Secreted. Post-translational modifications: O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. Similarity: Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain. SWISS: P24592 Gene ID: 3489 Database links: Entrez Gene: 3489 Human Entrez Gene: 16012 Mouse Omim: 146735 Human SwissProt: P24592 Human SwissProt: P47880 Mouse Unigene: 274313 Human Unigene: 358609 Mouse Unigene: 6431 Rat |
| 產(chǎn)品圖片 |
Sample: muscle (Mouse) Lysate at 40 ug
Primary: Anti- IGFBP6 (bs-4064R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 36 kD
Sample:
Brain (Rat) Lysate at 40 ug
Intestine (Mouse) Lysate at 40 ug
Primary: Anti-IGFBP6 (bs-4064R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP6 Polyclonal Antibody, Unconjugated(bs-4064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
