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Rabbit Anti-Adipose Triglyceride Lipase  antibody (bs-3831R)
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產(chǎn)品編號 bs-3831R
英文名稱 Rabbit Anti-Adipose Triglyceride Lipase  antibody
中文名稱 脂肪甘油三酯脂酶抗體
別    名 PEDF R; Adipose Triglyceride Lipase; TTS 2.2; PEDFR; PEDF-R; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Desnutrin; EC 3.1.1.3; IPLA2 zeta; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA; Pigment epithelium derived factor; Pnpla2; Transport secretion protein 2; Transport secretion protein 2.2; Triglyceride hydrolase; TTS2; TTS2.2; ZETA.  
Specific References  (2)     |     bs-3831R has been referenced in 2 publications.
[IF=2.742] Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7  WB ;  Mouse.  
[IF=2.532] Chenming Zhang. et al. Transcriptomics and proteomics analysis to explore the mechanism of Yishen Tongluo formula repairing sperm DNA damage in rats. ANDROLOGIA. 2022 Sep;:e14582  WB ;  Rat.  
研究領(lǐng)域 免疫學(xué)  生長因子和激素  糖尿病  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse (predicted: Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=3ug/Test,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55kDa
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ATGL: 401-504/504 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

Subunit:
Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity.

Subcellular Location:
Lipid droplet. Cell membrane.

Tissue Specificity:
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).

Similarity:
Contains 1 patatin domain.

SWISS:
Q96AD5

Gene ID:
57104

Database links:

Entrez Gene: 57104 Human

Entrez Gene: 66853 Mouse

Entrez Gene: 361676 Rat

Omim: 609059 Human

SwissProt: Q96AD5 Human

SwissProt: Q8BJ56 Mouse

SwissProt: P0C548 Rat

Unigene: 654697 Human

Unigene: 29998 Mouse

Unigene: 19196 Rat



Pathway: Glycerolipid metabolism; triacylglycerol degradation.
產(chǎn)品圖片
Sample: Lane 1: Mouse Brown fat tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-Adipose Triglyceride Lipase (bs-3831R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 52 kDa
Blank control: A431. Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (bs-3831R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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