| 產(chǎn)品編號 | bs-3513R |
| 英文名稱 | Rabbit Anti-Nur77 antibody |
| 中文名稱 | 孤兒核受體蛋白Nur77抗體 |
| 別 名 | GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10; Growth Factor Response Protein 1; Hbr1; HMR; Hormone Receptor; MGC9485; N10; N10 nuclear protein; NAK 1; NAK1; NGFIB; NP 10; NP10; NR4A1; Nuclear hormone receptor NUR/77; Nuclear Hormone Receptor TR3; Nuclear Receptor Subfamily 4 Group A Member 1; NUR77; Orphan nuclear receptor HMR; Orphan nuclear receptor NR4A1; ST 59; ST59; Steroid receptor TR3; TR 3; TR3; TR3 orphan receptor; Early response protein NAK1. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 染色質(zhì)和核信號 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 糖尿病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 66kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nur77/GFRP: 351-450/598 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] Subunit: Binds DNA as a monomer (By similarity). Interacts with GADD45GIP1. Subcellular Location: Nucleus. Tissue Specificity: Fetal muscle and adult liver, brain and thyroid. Similarity: Belongs to the nuclear hormone receptor family. NR4 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P22736 Gene ID: 3164 Database links: Entrez Gene: 3164 Human Entrez Gene: 15370 Mouse Omim: 139139 Human SwissProt: P22736 Human SwissProt: P12813 Mouse Unigene: 524430 Human Unigene: 119 Mouse Unigene: 10000 Rat 孤兒核受體蛋白Nur77是早期反應(yīng)轉(zhuǎn)錄因子之一，多種刺激因子如缺氧可誘導其迅速表達，Nur77參與調(diào)控細胞增殖、存活、分化等過程。這類核受體是體內(nèi)廣泛分布的配體依賴性的轉(zhuǎn)錄因子，通過調(diào)控基因協(xié)調(diào)表達，在機體的生長發(fā)育、新陳代謝，細胞分化及體內(nèi)許多生長發(fā)育過程中發(fā)揮重要作用，其功能障礙將導致一系列疾病的發(fā)生，如；癌癥、糖尿病、肥胖、不孕不育等等。 |
| 產(chǎn)品圖片 |
Sample:
Muscle(Mouse) Lysate at 40 ug
Primary: Anti-Nur77(bs-3513R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 66 kD
Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human HeLa cell lysates
Primary: Anti-Nur77 (bs-3513R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 73 kDa
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nur77) Polyclonal Antibody, Unconjugated (bs-3513R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nur77 Polyclonal Antibody, Unconjugated(bs-3513R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Nur77 antibody (bs-3513R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
