| 產品編號 | bs-3156R |
| 英文名稱 | phospho-GCN2 (Thr667) Rabbit pAb |
| 中文名稱 | 磷酸化蛋白激酶GCN2蛋白抗體 |
| 別 名 | GCN2(phospho T667); p-GCN2(phospho T667); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 181 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667: PG(p-T)PP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. Tissue Specificity: Widely expressed. Post-translational modifications: Autophosphorylated on threonine residues. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. SWISS: Q9P2K8 Gene ID: 440275 Database links: Entrez Gene: 440275 Human Entrez Gene: 27103 Mouse Omim: 609280 Human SwissProt: Q9P2K8 Human SwissProt: Q9QZ05 Mouse Unigene: 656673 Human Unigene: 217616 Mouse Unigene: 8333 Rat GCN2蛋白的作用；可能是將短期記憶變成長期記憶的一個主控調節(jié)因子， GCN2蛋白在長期記憶形成中功能的發(fā)現(xiàn)將可能幫助研究人員開發(fā)出提高記憶力喪失的病人提高記憶力。 |
| 產品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170/181 kD
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
