| 產品編號 | bs-2739R |
| 英文名稱 | PDPK1 Rabbit pAb |
| 中文名稱 | 3磷酸肌醇依賴性蛋白激酶1抗體 |
| 別 名 | 3 phosphoinositide Dependent Protein Kinase 1; hPDK 1; hPDK 1; PDK 1; PkB kinase; PkB kinase like gene 1; PkB like 1; PDPK1_HUMAN. |
| 研究領域 | 細胞生物 神經(jīng)生物學 信號轉導 細胞凋亡 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDK1: 251-350/556 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma. [provided by Alliance of Genome Resources, Nov 2021] Function: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. Subunit: Interacts with NPRL2. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. Tissue Specificity: Appears to be expressed ubiquitously. Post-translational modifications: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. Contains 1 PH domain. Contains 1 protein kinase domain. SWISS: O15530 Gene ID: 5170 Database links: Entrez Gene: 5170 Human Entrez Gene: 18607 Mouse Omim: 605213 Human SwissProt: O15530 Human SwissProt: Q9Z2A0 Mouse Unigene: 459691 Human Unigene: 10504 Mouse Unigene: 10905 Rat PDK1是蛋白激酶B(PKB)的上游激酶，通過與3,4,5三磷酸磷脂酰肌醇作用激活相鄰的PKB分子。PDK1能夠激活包含PKB在內的一系列的AGC激酶家族成員。從而調節(jié)細胞代謝，生長，擴散，生存，抗凋亡等諸多生理過程。 |
| 產品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-PDPK1 (bs-2739R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDPK1) Polyclonal Antibody, Unconjugated (bs-2739R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
