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NFAM1 Rabbit pAb (bs-2680R)  
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產(chǎn)品編號 bs-2680R
英文名稱 NFAM1 Rabbit pAb
中文名稱 NFAM1抗體
別    名 NFAT activation molecule 1 precursor; NFAT activation molecule 1 precursor; Calcinerin/NFAT activating ITAM containing protein; CNAIP; NFAT activation molecule 1; Calcineurin/NFAT-activating ITAM-containing protein; NFAT-activating protein with ITAM motif 1; NFAM1_HUMAN.  
Specific References  (2)     |     bs-2680R has been referenced in 2 publications.
[IF=6.543] Zhu Jianbing. et al. Extracellular Vesicle-Derived circITGB1 Regulates Dendritic Cell Maturation and Cardiac Inflammation via miR-342-3p/NFAM1. OXID MED CELL LONGEV. 2022;2022:8392313  WB ;  Human.  
[IF=4.14] Sambandam et al. NFAM1 signaling enhances osteoclast formation and bone resorption activity in Paget's disease of bone. (2017) Bone. 101:236-244  WB ;  Rat.  
研究領(lǐng)域 免疫學  染色質(zhì)和核信號  信號轉(zhuǎn)導  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse (predicted: Rat,Pig,Horse)
產(chǎn)品應用 Flow-Cyt=1μg /test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 30 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NFAM1: 81-180/270 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 NFAM1 activates cytokine gene promoters such as the IL13 and TNF-alpha promoters. It contains an immunoreceptor tyrosine-based activation motif (ITAM) and is thought to regulate the signaling and development of B-cells.

Function:
May function in immune system as a receptor which activates via the calcineurin/NFAT-signaling pathway the downstream cytokine gene promoters. Activates the transcription of IL-13 and TNF-alpha promoters. May be involved in the regulation of B-cell, but not T-cell, development. Overexpression activates downstream effectors without ligand binding or antibody cross-linking.

Subunit:
Interacts with ZAP70 and SYK. No direct interaction with the B-cell antigen receptor (BCR).

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Partially recruited to lipid rafts upon BCR stimulation.

Tissue Specificity:
Highly expressed in neutrophils, primary monocytes, mast cells, monocytic cell lines and lymphocytes. Also expressed in spleen B and T cells, and lung. Expressed at low level in non-immune tissue.

Post-translational modifications:
N-glycosylated.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain.

SWISS:
Q8NET5

Gene ID:
150372

Database links:

Entrez Gene: 150372 Human

Omim: 608740 Human

SwissProt: Q8NET5 Human

Unigene: 436677 Human



產(chǎn)品圖片
Blank control:U937. Primary Antibody (green line): Rabbit Anti-NFAM1 antibody (bs-2680R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:U937. Primary Antibody (green line): Rabbit Anti-NFAM1 antibody (bs-2680R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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