| 產(chǎn)品編號 | bs-2499R |
| 英文名稱 | Rabbit Anti-CD69 antibody |
| 中文名稱 | 活化誘導(dǎo)分子CD69抗體 |
| 別 名 | CLEC2C; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; EA1; BL-AC/P26;C-type lectin domain family 2 member C; CD69_HUMAN; |
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Specific References (3) | bs-2499R has been referenced in 3 publications.
[IF=12.121] Rouven Hoefflin. et al. HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice. Nat Commun. 2020 Aug;11(1):1-21 IHC ; Mouse.
[IF=10.711] Zihuan Luo. et al. Ex vivo anchored PD-L1 functionally prevent in vivo renal allograft rejection. BIOENG TRANSL MED. 2022 Apr 06 FC ; Rat.
[IF=4.12] Mokuda et al. CD1a+ survivin+ dendritic cell infiltration in dermal lesions of systemic sclerosis. (2015) Arthritis.Res.Ther. 17:275 IHC ; Human.
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| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 干細胞 糖尿病 細胞表面分子 自然殺傷細胞 淋巴細胞 t-淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 檢測分子量 | 27-33 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD69: 101-199/199 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011] Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. Subunit: Homodimer; disulfide-linked. Subcellular Location: Membrane; Single-pass type II membrane protein. Tissue Specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. Post-translational modifications: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. Similarity: Contains 1 C-type lectin domain. SWISS: Q07108 Gene ID: 969 Database links: Entrez Gene: 969 Human Entrez Gene: 12515 Mouse Omim: 107273 Human SwissProt: Q07108 Human SwissProt: P37217 Mouse Unigene: 208854 Human Unigene: 74745 Mouse Unigene: 23779 Rat CD69屬于C-型凝集素受體家族,也是NK細胞信號傳導(dǎo)基因復(fù)合體家族的一員,是一種Ⅱ型跨膜糖蛋白. |
| 產(chǎn)品圖片 |
Sample: U937 Cell Lysate at 30 ug
Primary: Anti- CD69 (bs-2499R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 34 kD
Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD69 (bs-2499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/27 kD
Observed band size: 30 kD
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)) . Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (bs-2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
