| 產(chǎn)品編號 | bs-2104R |
| 英文名稱 | TNFAIP1 Rabbit pAb |
| 中文名稱 | 腫瘤壞死因子α誘導(dǎo)蛋白1抗體 |
| 別 名 | tumor necrosis factor, alpha-induced protein 1-like; Tumor necrosis factor-alpha-induced protein 1; Tumor necrosis factor alpha induced protein 1(endothelial); BACD2_HUMAN; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; BTB/POZ domain-containing protein TNFAIP1; endothelial; hBACURD2; Protein B12; tnfaip1; Tumor necrosis factor alpha induced protein 1; TNF-IP 1; TNF-IP-1; TNF-IP1. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TNFAIP1: 231-316/316 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008]. Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity. Subcellular Location: Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes. Post-translational modifications: Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA. Similarity: Belongs to the BACURD family. Contains 1 BTB (POZ) domain. SWISS: Q13829 Gene ID: 7126 Database links: Entrez Gene: 7126 Human Entrez Gene: 21927 Mouse Omim: 191161 Human SwissProt: Q13829 Human SwissProt: O70479 Mouse Unigene: 76090 Human Unigene: 386774 Mouse Unigene: 18199 Rat TNFAIP1 protein是第一個被鑒定受腫瘤壞死因子α誘導(dǎo)的蛋白,實驗證明TNFAIP1 蛋白可能參與DNA復(fù)制、合成、細(xì)胞凋亡以及人類的各種疾病等重要生理過程，但還有一些功能不清。 |
| 產(chǎn)品圖片 |
Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TNFAIP1 Polyclonal Antibody, Unconjugated(bs-2104R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP1) Polyclonal Antibody, Unconjugated (bs-2104R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP1) Polyclonal Antibody, Unconjugated (bs-2104R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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