| 產(chǎn)品編號 | bs-1683R |
| 英文名稱 | CNR1 Rabbit pAb |
| 中文名稱 | 鈣粘蛋白相關(guān)的神經(jīng)受體1抗體 |
| 別 名 | CNR1_HUMAN; Cannabinoid receptor 1; CB-R; CB1; CANN6; CNR; CB1A; CB1R; CB1K5; |
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Specific References (2) | bs-1683R has been referenced in 2 publications.
[IF=6.799] Brenda L. K. Coles. et al. A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells. Lab Chip. 2021 Oct;: FC ; Human.
[IF=2.96] Xinna Wang. et al. Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model. Chin Med-Uk. 2020 Dec;15(1):1-12 IHC ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CNR-1: 401-472/472 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009] Function: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Subunit: Interacts (via C-terminus) with CNRIP1. Subcellular Location: Cell membrane. Tissue Specificity: Widely expressed. Similarity: Belongs to the G-protein coupled receptor 1 family. Gene ID: 1268 Database links: Entrez Gene: 1268 Human Entrez Gene: 12801 Mouse Omim: 114610 Human SwissProt: Q5IS73 Chimpanzee SwissProt: P21554 Human SwissProt: P47746 Mouse Unigene: 709067 Human Unigene: 75110 Human Unigene: 7992 Mouse Unigene: 222240 Rat Unigene: 89774 Rat CB1又稱大麻素受體1,可導(dǎo)致大麻素類物質(zhì)作用及代謝異常,從而影響胃腸動力及感覺. |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-CNR1 (bs-1683R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNR1) Polyclonal Antibody, Unconjugated (bs-1683R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat hippocampus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CNR1) Polyclonal Antibody, Unconjugated (bs-1683R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat liver);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min;Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CNR1) Polyclonal Antibody, Unconjugated (bs-1683R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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