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Rabbit Anti-Wnt3a  antibody (bs-1700R)  
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產(chǎn)品編號 bs-1700R
英文名稱 Rabbit Anti-Wnt3a  antibody
中文名稱 信號通路Wnt3a抗體
別    名 MGC119418; MGC119419; MGC119420; Protein Wnt 3a Precursor; Protein Wnt-3a; Wingless type MMTV integration site family member 3A; Wnt 3a; WNT3A protein.  
Specific References  (17)     |     bs-1700R has been referenced in 17 publications.
[IF=13.273] Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094  WB ;  Mouse.  
[IF=13.12] B?scke, Robert, et al. "Wnt Signaling in Chronic Rhinosinusitis with Nasal Polyps." American Journal of Respiratory Cell and Molecular Biology ja (2017).  IF(ICC) ;  Human.  
[IF=5.97] Sun et al. miR-491-5p, mediated by Foxi1, functions as a tumor suppressor by targeting Wnt3a/β-catenin signaling in the development of gastric cancer. (2017) Cell.Death.Dis. 8:e2714  IHC,WB ;  Human.  
[IF=4.486] Xu T et al. MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med . 2020 Jul;24(13):7127-7140.  WB ;  Human.  
[IF=4.225] Yang Fan. et al. Catalpol Protects Against Pulmonary Fibrosis Through Inhibiting TGF-β1/Smad3 and Wnt/β-Catenin Signaling Pathways. Front Pharmacol. 2021 Jan;11:2472  WB,IHC ;  Rat.  
[IF=3.361] Ding Z et al. The CRD of Frizzled 7 exhibits chondroprotective effects in osteoarthritis via inhibition of the canonical Wnt3a/β-catenin signaling pathway. Int Immunopharmacol. 2020 Mar 6;82:106367.  IHC,WB ;  human&mouse.  
[IF=2.742] Cai Zhijun. et al. Overloaded axial stress activates the Wnt/β-Catenin pathway in nucleus pulposus cells of adult degenerative scoliosis combined with intervertebral disc degeneration. MOL BIOL REP. 2023 Apr;:1-8  WB ;  Human.  
[IF=2.698] Yanyan Xing. et al. Effects of mechanosensitive ion channel Piezo1 on proliferation and osteogenic differentiation of human dental follicle cells. Ann Anat. 2021 Oct;:151847  WB ;  Human.  
[IF=2.47] Zhu, Afang, et al. "Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats." Biochemical and Biophysical Research Communications (2017).  WB ;  Rat.  
[IF=2.454] Dobzanski?A et al. Nasal polyp fibroblasts modulate epithelial characteristics via Wnt signaling. Int Forum Allergy Rhinol. 2018 Dec;8(12):1412-1420.   ICF ;  Human.  
[IF=2.37] Hayashi, Kouhei, et al. "Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland." Biochemical and Biophysical Research Communications (2016).  WB ;  Mouse.  
[IF=2.089] Huan He. et al. BDE-209 disturbed proliferation and differentiation of spermatogonia during mitotic process through estrogen receptor α. REPROD BIOL. 2023 Jun;23:100737  WB,IF ;  Rat.  
[IF=1.92] Yang et al. MicroRNA-195 acts as a tumor suppressor by directly targeting Wnt3a in HepG2 hepatocellular carcinoma cells. (2014) Mol.Med.Rep. 10:2643-8  WB ;  Human.  
[IF=1.48] Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular Medicine Reports.  other ;  
[IF=1.43] Jin, Xinhao, et al. "Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 207.  WB ;  Rat.  
[IF=1.26] Guo, Furong, et al. "Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease." Experimental and Therapeutic Medicine.(2017)  WB ;  Human.  
[IF=1.03] Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7.  IHC-P ;  Rat.  
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  干細(xì)胞  細(xì)胞分化  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 37 kDa
檢測分子量
細(xì)胞定位 細(xì)胞外基質(zhì) 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Wnt3a: 281-352/352 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Function:
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Subunit:
Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6.

Subcellular Location:
Secreted, extracellular space, extracellular matr

Tissue Specificity:
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.

Post-translational modifications:
Palmitoylation at Ser-209 by PORCN is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoylated WNT3A from WLS in secretory vesicles.

Similarity:
Belongs to the Wnt family.

SWISS:
P56704

Gene ID:
89780

Database links:

Entrez Gene: 89780 Human

Entrez Gene: 22416 Mouse

Entrez Gene: 303181 Rat

Omim: 606359 Human

SwissProt: P56704 Human

SwissProt: P27467 Mouse

Unigene: 336930 Human

Unigene: 1367 Mouse

Unigene: 130971 Rat



Wnt3a是Wnt家族的成員之一,對神經(jīng)干細(xì)胞的增殖及分化有一定的調(diào)控作用,Wnt3a蛋白可以促進(jìn)神經(jīng)干細(xì)胞向神經(jīng)元分化.
產(chǎn)品圖片
Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug Lane 2: Stomach (Rat) Lysate at 40 ug Primary: Anti-Wnt3a (bs-1700R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD
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