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HP1 gamma Rabbit pAb (bs-1421R)  
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產(chǎn)品編號(hào) bs-1421R
英文名稱 HP1 gamma Rabbit pAb
中文名稱 異染色質(zhì)蛋白1-γ/HP-1γ抗體
別    名 CBX 3; CBX3; Chromobox homolog 3; Chromobox protein homolog 3; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma; HP1 gamma homolog; HP1Hs gamma; Modifier 2; protein HP1-GAMMA; HP1g; M32; MGC118084; CBX3_HUMAN; Modifier 2 protein;  HP-1-γ; HP 1γ; HP-1γ; HP-1 γ; HP1γ; HP1 γ;
研究領(lǐng)域 腫瘤  細(xì)胞生物  染色質(zhì)和核信號(hào)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 21 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HP1 gamma: 10-150/183 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Function:
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Subunit:
Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1.

Subcellular Location:
Nucleus (Potential). Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).

Post-translational modifications:
Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

Similarity:
Contains 2 chromo domains.

SWISS:
Q13185

Gene ID:
11335

Database links:

Entrez Gene: 11335 Human

Entrez Gene: 12417 Mouse

Entrez Gene: 297093 Rat

Omim: 604477 Human

SwissProt: Q13185 Human

SwissProt: P23198 Mouse

Unigene: 381189 Human

Unigene: 280968 Mouse

Unigene: 458234 Mouse

Unigene: 485141 Mouse



產(chǎn)品圖片
Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HP1 gamma Polyclonal Antibody, Unconjugated(bs-1421R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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