| 產(chǎn)品編號 | bs-0679R |
| 英文名稱 | Rabbit Anti-SYVN1 antibody |
| 中文名稱 | 滑膜細(xì)胞凋亡抑制物1抗體 |
| 別 名 | 1200010C09Rik; HRD1; KIAA1810; MGC40372; Synovial apoptosis inhibitor 1 synoviolin; Synoviolin 1 isoform b; SYVN1_HUMAN; DER3; E3 ubiquitin-protein ligase synoviolin; HMG coA reductase degradation 1 homolog; OTTHUMP00000230429; OTTHUMP00000230430; OTTHUMP00000230431; OTTHUMP00000230432; Synovial apoptosis inhibitor 1; Synoviolin 1 isoform b; SYNOVIOLIN; SYVN1. |
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Specific References (4) | bs-0679R has been referenced in 4 publications.
[IF=5.064] Dong J et al. Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathwayActa Pharmacol Sin.2020 Dec 2. IHC、IF ; Mouse.
[IF=4.65] Zemoura, Khaled, et al. "Endoplasmic Reticulum Associated Degradation (ERAD) Controls Cell Surface Expression of GABAB Receptors." Journal of Biological Chemistry (2013): jbc-M113. Human.
[IF=4.26] Yang et al. Experimental study of the protective effects of SYVN1 against diabetic retinopathy. (2015) Sci.Re. 5:14036 WB ; Mouse.
[IF=1.89] Yan, Shu, et al. "Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats." Experimental diabetes research 2012 (2011). Rat.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=3μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SYVN1: 531-617/617 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011] Function: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Subunit: Homodimer. Interacts with p53/TP53 and HTT. Interacts with VCP, HERPUD1 and DERL1. Part of a complex containing SYVN1, HERPUD1, SELS and DERL1; which probably transfer misfolded proteins from the ER to VCP. Part of a complex containing SYVN1, SEL1L and DERL2. Interacts with UBXN6. Interacts with SEL1L; recruits ERLEC1 and OS9. May form a complex with ERLEC1; HSPA5; OS9 AND SEL1L. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level). Post-translational modifications: Not N-glycosylated. Auto-ubiquitinated. Similarity: Belongs to the HRD1 family. Contains 1 RING-type zinc finger. SWISS: Q86TM6 Gene ID: 84447 Database links: Entrez Gene: 84447 Human Entrez Gene: 74126 Mouse Omim: 608046 Human SwissProt: Q86TM6 Human SwissProt: Q9DBY1 Mouse Unigene: 75859 Human Unigene: 149870 Mouse 與關(guān)節(jié)滑膜的損傷有關(guān)。 |
| 產(chǎn)品圖片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti-SYVN1 (bs-0679R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65/70 kD
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SYVN1) Polyclonal Antibody, Unconjugated (bs-0679R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
The figure annotation:
The blue histogram is unstained cells.
The Wathet Blue histogram is cells stained with secondary antibody (bs-0295G-FITC) alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody(bs-0295P)plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-SYVN1 antibody (bs-0679R) plus secondary antibody.
Positive control: Hepg2 cells
Concentration: 1:50
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