| 產(chǎn)品編號 | bs-0823R |
| 英文名稱 | Granulin Rabbit pAb |
| 中文名稱 | 顆粒蛋白前體抗體 |
| 別 名 | Granulin; Acrogranin; GEP; GP88; Granulin epithelin; GRN; PC cell derived growth factor; PCDGF; PEPI; PGRN; Proepithelin; Progranulin. |
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Specific References (2) | bs-0823R has been referenced in 2 publications.
[IF=7.02] Thurner, Lorenz, et al. "The molecular basis for development of proinflammatory autoantibodies to progranulin." Journal of Autoimmunity (2015). WB ; Human.
[IF=4.238] Dongxu Feng. et al. Progranulin modulates cartilage-specific gene expression via sirtuin 1–mediated deacetylation of the transcription factors SOX9 and P65. J Biol Chem. 2020 Sep;295:13640 WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PGNR: 451-550/593 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Function: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Subcellular Location: Secreted. Tissue Specificity: In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney. Post-translational modifications: Granulins are disulfide bridged. DISEASE: Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. Similarity: Belongs to the granulin family. SWISS: P28799 Gene ID: 2896 Database links: Entrez Gene: 2896 Human Entrez Gene: 14824 Mouse Omim: 138945 Human SwissProt: P28799 Human SwissProt: P28798 Mouse Unigene: 514220 Human Unigene: 1568 Mouse 1.PGRN目前主要用于老年癡呆疾病。是65歲以下人群中第二種最常見的 癡呆形式，該蛋白的多量產(chǎn)生，能夠損壞記憶和個性，而且還可能影響運動。這一發(fā)現(xiàn)也許可幫助解決關(guān)于該疾病病因的困惑： 癡呆癥(FTD)是常見的神經(jīng)退化疾病，可見，除微管相關(guān)的tau蛋白導(dǎo)致癡呆癥外，又一個新的與癡呆癥有關(guān)蛋白又被科學(xué)家發(fā)現(xiàn)。 2.顆粒蛋白前體（PGRN）在調(diào)節(jié)生長發(fā)育、損傷修復(fù)和促進(jìn)細(xì)胞增殖等方面發(fā)揮重要作用。PGRN能促進(jìn)腫瘤細(xì)胞過度增殖、增強(qiáng)細(xì)胞向外周侵襲的能力以及促進(jìn)腫瘤組織血管新生，提示它可能是腫瘤治療的一個非常重要的靶標(biāo)。 |
| 產(chǎn)品圖片 |
Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PGRN/Granulin Polyclonal Antibody, Unconjugated(bs-0823R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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