| 產(chǎn)品編號 | bs-0829R |
| 英文名稱 | Nanog Rabbit pAb |
| 中文名稱 | 胚胎干細胞關(guān)鍵蛋白抗體 |
| 別 名 | NANOG_MOUSE; Embryonic stem cell specific homeobox protein(Nanog); ENK; FLJ12581; FLJ40451; Homeobox transcription factor Nanog; Nanog homeobox; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog; Ecat4. |
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Specific References (11) | bs-0829R has been referenced in 11 publications.
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| 研究領(lǐng)域 | 腫瘤 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 干細胞 細胞周期蛋白 細胞表面分子 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,Flow-Cyt=3μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Nanog: 71-170/305 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm). Function: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Subunit: Interacts with SMAD1 and SALL4. Subcellular Location: Nucleus. Tissue Specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Similarity: Belongs to the Nanog homeobox family. Contains 1 homeobox DNA-binding domain. Gene ID: 71950 Database links: Entrez Gene: 79923 Human Entrez Gene: 71950 Mouse Omim: 607937 Human SwissProt: Q9H9S0 Human Unigene: 635882 Human 研究表明；早期胚胎在沒有Nanog存在的情況下是不能存活的，但是這一蛋白的表達水平至今還有待于進一步研究。學(xué)者認為：Nanog依然是一種對于生殖細胞（germ lls）形成的必要因子，Nanog也是調(diào)控著細胞融合后的多重功能的關(guān)鍵因子。 胚胎干細胞是當今生命科學(xué)和生物技術(shù)研究的熱點，這主要是由于其具有“發(fā)育全能性”的功能。ES能分化成人體200多種細胞類型，形成機體的任何細胞、組織和器官。通過掌握其分化發(fā)育的規(guī)律，在人工條件下定向分化為所需的細胞、組織乃至器官，科學(xué)家們希望可以用來治療目前還難以或無法治愈的帕金森氏病、早老性癡呆、白血病、糖尿病等頑癥，并且解決十分緊缺的組織和器官移植的來源問題，并且通過進一步與克隆技術(shù)相結(jié)合，運用體細胞核轉(zhuǎn)移技術(shù)來得到ES，還能解決細胞治療以及組織和器官移植的免疫排異難題。 |
| 產(chǎn)品圖片 |
Tissue/cell: Mouse tooth germ tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated(bs-0829R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-Nanog antibody(bs-0829R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
